Hi all,
I'm currently using the Covaris S2 to fragment my DNA to approximately 1.5-2kb.
Mainly so I can ligate to perform an inverse PCR before NGS library prep. My problem seems to be when I need to analyse the fragment pattern on a gel. As I only have 130ul I struggle to add enough to visualise using ethidium bromide.
is it possible to fragment a higher concentration, i've read its independent but wondered if sonicating for a longer time would have a detrimental effect on my DNA.
Are there any other ways round this??
Many thanks
Adam
I'm currently using the Covaris S2 to fragment my DNA to approximately 1.5-2kb.
Mainly so I can ligate to perform an inverse PCR before NGS library prep. My problem seems to be when I need to analyse the fragment pattern on a gel. As I only have 130ul I struggle to add enough to visualise using ethidium bromide.
is it possible to fragment a higher concentration, i've read its independent but wondered if sonicating for a longer time would have a detrimental effect on my DNA.
Are there any other ways round this??
Many thanks
Adam
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