Out of curiosity, and with the TruSeq v2 Y-adaptor chemistry:
If you have sufficient amounts of adapted DNA, how important is it to do the PCR enrichment? In principle, my expectation is that cluster generation will work without enrichment since the adaptors should be ligated on both strands. In not a few instances, I've been getting yields upwards to 0.5 nM of adapted DNA, which is more than enough for putting on the Miseq.
Thoughts?
If you have sufficient amounts of adapted DNA, how important is it to do the PCR enrichment? In principle, my expectation is that cluster generation will work without enrichment since the adaptors should be ligated on both strands. In not a few instances, I've been getting yields upwards to 0.5 nM of adapted DNA, which is more than enough for putting on the Miseq.
Thoughts?
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