Hi,
I constructed an Illumina paired-end library for test, and TOPO cloned an aliquot of the library. I just received six sequence of the picked clones, all of them are adaptered fragments with correct insert size. BUT, only one of the insert fragment are from the genome of my organism. I try to find where are the others from, but I can't find any significant hits using NCBI blastn against nt database. They don't look like dimer, as they are not identical to each other.
Is there anyone have similar experience with this? I know they might be contamination, but I don't know what types of contamination are they, and why they dominate my library?
I constructed an Illumina paired-end library for test, and TOPO cloned an aliquot of the library. I just received six sequence of the picked clones, all of them are adaptered fragments with correct insert size. BUT, only one of the insert fragment are from the genome of my organism. I try to find where are the others from, but I can't find any significant hits using NCBI blastn against nt database. They don't look like dimer, as they are not identical to each other.
Is there anyone have similar experience with this? I know they might be contamination, but I don't know what types of contamination are they, and why they dominate my library?
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