DNA Methylation Data Analysis Workshop - 2-5 November 2020 in Berlin

[ecSeq Bioinformatics]

How to use bisulfite-treated sequencing to study DNA methylation
Advance your epigenetics research with NGS methods.

Link to workshop website

In a nutshell

• Learn how bisulfite sequencing works
• Understand how bisulfite-treated reads are mapped to a reference genome
• Perform basic analyses (call methylated regions, perform basic downstream analyses)
• Use shell scripting to create reusable data pipelines
• Visualize results (ready-to-publish)

The purpose of this workshop is to get a deeper understanding of the use of bisulfite-treated DNA in order to analyze the epigenetic layer of DNA methylation. Advantages and disadvantages of the so-called 'bisulfite sequencing' and its implications on data analyses will be covered. The participants will be trained to understand bisulfite-treated NGS data, to detect potential problems/errors and finally to implement their own pipelines. After this course they will be able to analyze DNA methylation and create ready-to-publish graphics.

By the end of this workshop the participants will:

• be familiar with the sequencing method of Illumina
• understand how bisulfite sequencing works
• be aware of the mapping problem of bisulfite-treated data
• understand how bisulfite-treated reads are mapped to a reference genome
• be familiar with common data formats and standards
• know relevant tools for data processing
• automate tasks with shell scripting to create reusable data pipelines
• perform basic analyses (call methylated regions, perform basic downstream analyses)
• plot and visualize results (ready-to-publish)
• be able to reuse all analyses

More information here