Mi-Seq Run Time?

bwking82 · 01-09-2012, 08:46 AM

Hello all,

Just a little bit curious to hear from someone who has actually used the Mi-Seq. I'm working with a group that is considering some samples off to be run on a Mi-Seq, but we don't have much information on instrument run times.

Using this released run data from Illumina

1 x 35 bp 4 hours
2 x 100 bp 19 hours
2 x 150 bp 27 hours

I have determined that the cycle time is a little under 5 minutes and the SE cluster generation is about an 1.2 hours and the total PE cluster time is 3 hours. Can anyone with field experience confirm or debunk these approximations? I have seen a post on a facility's website that advertised 2 x 25 reads being ran in about 5 hour run times, which jives with my math and tells me I may be missing something. Any help would be appreciated

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Tophat Run time	bassu	Bioinformatics	11	09-17-2013 10:59 PM
RNA seq with two time points	anle	Bioinformatics	0	01-16-2012 01:24 AM
RNA-seq analysis for time course	honey	Bioinformatics	0	01-18-2011 12:56 PM
TopHat approximate run time & memory usage?	xinchen	Bioinformatics	4	05-18-2010 03:47 AM
SOLiD 4 run time	Bruins	SOLiD	3	03-04-2010 01:34 AM

01-09-2012, 08:46 AM	#1
bwking82 Junior Member Location: RTP Join Date: Sep 2008 Posts: 2	Mi-Seq Run Time? Hello all, Just a little bit curious to hear from someone who has actually used the Mi-Seq. I'm working with a group that is considering some samples off to be run on a Mi-Seq, but we don't have much information on instrument run times. Using this released run data from Illumina 1 x 35 bp 4 hours 2 x 100 bp 19 hours 2 x 150 bp 27 hours I have determined that the cycle time is a little under 5 minutes and the SE cluster generation is about an 1.2 hours and the total PE cluster time is 3 hours. Can anyone with field experience confirm or debunk these approximations? I have seen a post on a facility's website that advertised 2 x 25 reads being ran in about 5 hour run times, which jives with my math and tells me I may be missing something. Any help would be appreciated