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Old 01-09-2012, 08:46 AM   #1
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Location: RTP

Join Date: Sep 2008
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Default Mi-Seq Run Time?

Hello all,

Just a little bit curious to hear from someone who has actually used the Mi-Seq. I'm working with a group that is considering some samples off to be run on a Mi-Seq, but we don't have much information on instrument run times.

Using this released run data from Illumina

1 x 35 bp 4 hours
2 x 100 bp 19 hours
2 x 150 bp 27 hours

I have determined that the cycle time is a little under 5 minutes and the SE cluster generation is about an 1.2 hours and the total PE cluster time is 3 hours. Can anyone with field experience confirm or debunk these approximations? I have seen a post on a facility's website that advertised 2 x 25 reads being ran in about 5 hour run times, which jives with my math and tells me I may be missing something. Any help would be appreciated
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