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Thread | Thread Starter | Forum | Replies | Last Post |
intersectBed (BEDtools) generating empty output file | palc | Bioinformatics | 1 | 08-28-2012 09:36 AM |
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BEDTools v2.6: improved BAM support, new options. | quinlana | Bioinformatics | 3 | 03-31-2011 12:41 PM |
BEDTools support for BAM/GFF/BED12 | quinlana | Bioinformatics | 1 | 01-12-2010 01:20 AM |
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#1 |
Just a member
Location: Southern EU Join Date: Nov 2012
Posts: 103
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Hi all,
I want to discard the reads that overlap some blacklist regions, so I use intersectbed -abam -v -a readfile.bam -b badregions.bed I get 9 entries in BED format, despite the abam option ("The A input file is in BAM format. Output will be BAM as well.") Now if I add the -wa option I get 3.5M entries, again in BED format. Any idea about what is going on? thanks |
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#2 |
Just a member
Location: Southern EU Join Date: Nov 2012
Posts: 103
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OK, the command line was wrong; it should have been
intersectbed -v -abam readfile.bam -b badregions.bed problem solved ![]() |
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