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Thread | Thread Starter | Forum | Replies | Last Post |
FastQC,kmer content, per base sequence content: is this good enough | mgg | Bioinformatics | 10 | 11-06-2013 11:45 PM |
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Kmer content | subuhikhan | General | 9 | 03-05-2012 01:05 AM |
kmer content in the first bases of Illumina sequence | brachysclereid | Bioinformatics | 2 | 01-09-2012 03:54 PM |
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#1 |
Member
Location: Michigan Join Date: Jul 2011
Posts: 10
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I would be so grateful if someone wouldn't mind taking a look at the kmer content graph from FastQC. This is RNA-seq data after trimming and filtering based on quality scores. My questions are: Does the y-axis represent frequency? If it does why are these kmers so frequently found? What could they be? Transposons? DNA? I should mention, that there are no overrepresented sequences detected by FastQC.
Thank you in advance for your time!!! |
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