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09-13-2018, 11:16 PM
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Junior Member
Location: italy Join Date: Sep 2018
Posts: 2
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Methylation: Igv and bismark question
Hi i have a question, i working for first time with bismark, after alignment i watched the results on IGV. Why each column methylated contains cytosine and tymine? spontaneous deamination? (Watch image attached)
I have file paired-end and i use this command line: bismark --multicore 6 --non_directional /genomes/ -1 file_R1.fq.gz -2 file_R2.fq.gz why the file .bam seems a Single-end alignment?? thank you Giuseppe |
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