Hello everybody
I'm analyzing three metagenomic samples from viruses extracted from river waters.
Before sequencing samples underwent tangential flow filtration step/ CsCl ultracentrifugation, Dnase treatment and check with qPRC.
In only one of the three samples I found a massive presence of human reads (around 80%, the other 20% were viral and bacterial reads), while very little presence was observed in the other two samples (< 2%).
The BLAST analysis of these reads usually give >95% identity against human.
Can anyone suggest me an explaination?
Thank you to everybody
I'm analyzing three metagenomic samples from viruses extracted from river waters.
Before sequencing samples underwent tangential flow filtration step/ CsCl ultracentrifugation, Dnase treatment and check with qPRC.
In only one of the three samples I found a massive presence of human reads (around 80%, the other 20% were viral and bacterial reads), while very little presence was observed in the other two samples (< 2%).
The BLAST analysis of these reads usually give >95% identity against human.
Can anyone suggest me an explaination?
Thank you to everybody
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