Tweet
Does anyone know what the SOLiD 3plus upgrade entails?
-
-
<Original post Deleted since we haven't been through the 3+ upgrade yet>.
Notes from my co-worker (who was out at the recent SOLiD meeting): Not much of machine change this time, just a lower-profile flowcell. He also reports a 50% increase in beads per flowcell and that v.3 chemistry will run on the v.3.5 machines. -
This was discussed at length at the SOLiD User's summit last week. With respect to what Rick wrote, a new bead finding algorithm will allow a roughly 50% increase in the number of beads per flowcell. The recommended bead density will increase from 130-150 thousand beads/panel to 220 thousand beads/panel. Or from roughly 330 million raw reads/slide to 500 million.Originally posted by NextGenSeq View Post
This is accompanies a flowcell swap out, new slide carriers and a newly optimized sequence master mix. I remember there being a slight decrease in chemistry cost, although my guess is that it is more than counteracted by the extra ePCR that will be necessary to generate all those beads...
Perhaps more importantly a major secondary/tertiary software release will happen in "early November" called "BioScope v 1.0". Briefly, this package will replace Global SETS, but be free for SOLiD owners (unlike Global SETS). Mapping is said to be 3-10x faster and utilize a "seeded extension mapping" method that is said to successfully map 10-30% more reads.
The really crazy stuff seems to be in version 4, which is slated for "2010".
--
PhillipComment
-
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
Comment