Hi all,
I'm working on bacteria.
I received the full sequence of a microrganism. The genome was 9Mb in length.
I performed several tests on this microrganisms (they took 2 years) and now we want to know if these tests were responsible for changes in a specific region of the genome (0.2Mb).
I'm a newbie in sequencing so I'd like to ask if the targeted resequencing could be useful for me!
What do you think?
Additional question:
I read this sentence on Illumina website:
"Regions of interest are captured by hybridization to biotinylated probes and then isolated by magnetic pulldown. Target enrichment captures 20 kb–62 Mb regions, depending on the experimental design."
but I didn't find a lots of paper using this technique on bacteria genomes... so is there something that I'm not considering about this technique that does not allow me to use it for my purpose?
I'd really appreciate any help.
Thanks
I'm working on bacteria.
I received the full sequence of a microrganism. The genome was 9Mb in length.
I performed several tests on this microrganisms (they took 2 years) and now we want to know if these tests were responsible for changes in a specific region of the genome (0.2Mb).
I'm a newbie in sequencing so I'd like to ask if the targeted resequencing could be useful for me!
What do you think?
Additional question:
I read this sentence on Illumina website:
"Regions of interest are captured by hybridization to biotinylated probes and then isolated by magnetic pulldown. Target enrichment captures 20 kb–62 Mb regions, depending on the experimental design."
but I didn't find a lots of paper using this technique on bacteria genomes... so is there something that I'm not considering about this technique that does not allow me to use it for my purpose?
I'd really appreciate any help.
Thanks
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