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Just annealing two single strand DNA? Is there any following purification steps needed? like ethanol precipitation?
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No there is no need for further treatment. Two very good descriptions of the process are the Illumina patent ((WO/2008/015396) METHOD OF LIBRARY PREPARATION AVOIDING THE FORMATION OF ADAPTOR DIMERS)) and the supplementary methods for the paper Identification of genetic variants using bar-coded multiplexed sequencing Nat Methods.2008 Oct;5(10):887-*‐93.Originally posted by chromatin View Post
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
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