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Old 07-13-2012, 06:42 AM   #1
Chuckytah
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Location: Barcelos, Braga, Portugal

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Angry From Fasta get GO-terms

Hello friends,

I used to do GO annotation using Blast2GO but it strangely gave me 0 GO-terms to a 507 sequences dataset... it was really strange... The blast went fine, but when I try the annotation it gives me no hits at all... I dunno if I made me clear.

You know any other software to have GO-terms from fasta files?

Thanks.
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Old 07-15-2012, 07:26 AM   #2
JackieBadger
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did you change the type of blast search? GO annotations only work with BLASTx.... tBLASTx does not work
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Old 07-16-2012, 04:51 AM   #3
Chuckytah
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Quote:
Originally Posted by JackieBadger View Post
did you change the type of blast search? GO annotations only work with BLASTx.... tBLASTx does not work
I really can't remember if I did any alteration in the parameters. lol but I already solve my problem in tab Tools there is an option that "Restore all original B2G properties", and now everything is okay.
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blast, gene annotation, gene ontology, go terms

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