SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
Has the NextSeq forgotten how to use custom primers? jwfoley Illumina/Solexa 2 04-08-2015 10:35 PM
Miseq: ERROR: FlowCell ID is inconsistent across Sample Sheet lines. harijay Illumina/Solexa 1 02-13-2015 04:12 PM
Free app. for sample tracking and sample sheet generation aubreeh Illumina/Solexa 0 10-15-2013 01:00 PM
.csv sample sheet for Caporaso protocol -16s Amplicon Sequencing chiaraf Sample Prep / Library Generation 0 02-26-2013 01:25 AM
sample sheet? babilei 454 Pyrosequencing 1 01-05-2011 05:42 AM

Reply
 
Thread Tools
Old 02-19-2018, 07:48 AM   #1
Beta10
Junior Member
 
Location: Ireland

Join Date: Feb 2017
Posts: 1
Default Sample sheet for NextSeq-custom

hi,

i have a single index run of 16 samples i want to run on the NS. These are not my samples and i was provided with 2 sets of indexes for these samples as they are not too sure what ones they used in setting up the library prep!!!!!

Anyhow, what can i do? i now have a choice of 32 indexes for my 16 samples. Can i set up my sample sheet to reflect this? Its a custom set of indexes.

Thanks!
Beta10 is offline   Reply With Quote
Old 02-19-2018, 09:44 AM   #2
GenoMax
Senior Member
 
Location: East Coast USA

Join Date: Feb 2008
Posts: 6,783
Default

You could just use sample1_index, sample2_index sample32_index type thing to set up a samplesheet to do the demux. Once you know what indexes are real you can do a second run with sampleNames_indexes to clean things up. Ultimately the submitters would need to know what index goes with what sample since there is no way to figure that out automagically.
GenoMax is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 09:04 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO