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Old 01-04-2018, 02:05 PM   #1
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Default Library prep for FFPE samples /Illumina

I am looking for library prep kits compatible with illumina NextSeq/NovaSeq platform. I have RNA and DNA from FFPE samples and am looking for kits for both RNASeq and exome sequencing.

Could you please guide me on the most cost effective and high quality kit.

I am thinking about using KAPA/ROCHE HYPERPLUS kit for exome sequencing.

I am debating between

TruSeq Stranded Total RNA Library Prep by illimina or TrueSeq RNA ACCESS by illumina for RNASeq.

Q.1. What is the difference between these two kits?
Q.2. I do not have a germline control at the moment. What can I do about that?
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Old 01-17-2018, 11:07 PM   #2
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I am using the RNA access kit. It is better for degraded samples.
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Old 03-10-2018, 12:24 PM   #3
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Default QuantSeq 3' mRNA-seq lib prep kit for degraded RNA

Hi, for gene expression analysis please have a look at the QuantSeq kit from Lexogen:

Due to it's focus on 3' ends this established method shows excellent gene expression determination perfomance with heavily degraded and FFPE-derived RNA - from USD 19.80 per sample incl. data analysis!

If you have any further questions, please e-mail [email protected] and have a look at Fig. 7 and Fig. 8 of the QuantSeq Application Note.

Lukas Paul
Sr. Manager of Scientific Affairs
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