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Old 09-01-2019, 09:26 AM   #1
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Location: Connecticut

Join Date: Aug 2019
Posts: 1
Default Non-Barcoded reads. Why?

Hello everybody!

I am pretty new in the NGS jungle and I am struggling with the making sense of some NGS data. I hope someone can help me out:

I have built a library with internal Barcodes which looks like the following:
N=random nucleotides to collapse PCR duplicates

Unfortunately, after Barcode splitting, I noticed that I have many reads with no Barcode.

Does any of you have an explanation why I get such non-barcoded reads?

Any input would be much appreciated

Thanks in advance!

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