Dear All,
atm i'm trying do preprocess RNA Seq data for the Use of FusionSeq with
RSEQ tools:
The step in which the reads are Aligned against the Splice Junction Library
with bowtie totally fails with output:
# reads processed: 1000000
# reads with at least one reported alignment: 0 (0.00%)
# reads that failed to align: 1000000 (100.00%)
No alignments
while mapping the reads against the genome works fine.
I checked the content of the splice Junction Library, mapping it and receiving partial alignments (with BLAT).
Does anyone have an idea what is going wrong here?
Thanks in Advance,
DIV
atm i'm trying do preprocess RNA Seq data for the Use of FusionSeq with
RSEQ tools:
The step in which the reads are Aligned against the Splice Junction Library
with bowtie totally fails with output:
# reads processed: 1000000
# reads with at least one reported alignment: 0 (0.00%)
# reads that failed to align: 1000000 (100.00%)
No alignments
while mapping the reads against the genome works fine.
I checked the content of the splice Junction Library, mapping it and receiving partial alignments (with BLAT).
Does anyone have an idea what is going wrong here?
Thanks in Advance,
DIV