Hello all,
I am a little bit perplexed of the exact mening of the term 'channel' as it is used by Helicos. So, I understand that there machine has two flow cells - in one the synthesis reaction takes place whilst the other is being imaged and this switches back and forth for each added nucleotide. However, each flow cell has 25 independent channels; what does that mean? Does each channel correspond to a picture like this one? Basically, if the HeliScope was a qPCR machine, would it correspond to it being able to run two multiwell plates at the time, with 25 wells per plate?
Edit: Just found a footnote in their product specification sheet stating that for more than 50 samples multiplexing or barcoding is required. This would mean that I am right, right?
I am a little bit perplexed of the exact mening of the term 'channel' as it is used by Helicos. So, I understand that there machine has two flow cells - in one the synthesis reaction takes place whilst the other is being imaged and this switches back and forth for each added nucleotide. However, each flow cell has 25 independent channels; what does that mean? Does each channel correspond to a picture like this one? Basically, if the HeliScope was a qPCR machine, would it correspond to it being able to run two multiwell plates at the time, with 25 wells per plate?
Edit: Just found a footnote in their product specification sheet stating that for more than 50 samples multiplexing or barcoding is required. This would mean that I am right, right?
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