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Anyone done it or thought about it?
I'm very interested in finding out if the gaps in my coverage are caused by the baits just not being efficiently synthesized/transcribed. This is one explanation for the LOW variability in baits from the same array printing, but HIGH variability between different printings of the same content.
I know I could probably do individual RT-PCRs, but I'd prefer to just massively query the whole bait population.
I suppose the bio-UTP could affect RT activity, and it's very possible Im missing something else...but just wanted to throw this out there for discussion.
I'm very interested in finding out if the gaps in my coverage are caused by the baits just not being efficiently synthesized/transcribed. This is one explanation for the LOW variability in baits from the same array printing, but HIGH variability between different printings of the same content.
I know I could probably do individual RT-PCRs, but I'd prefer to just massively query the whole bait population.
I suppose the bio-UTP could affect RT activity, and it's very possible Im missing something else...but just wanted to throw this out there for discussion.
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