Hi everybody, im working with a De NOVO plant assembly and i used two differents Assemblers, Mira and Masurca, i got a problem with how to know the real coverage in the MaSurCa analisys, because Masurca made super reads with the reads for make the assembly genome and like the literature say, this one is between 2.X an 4.X for a high coverage Illumina sequencing. My question is if somebody how to calculate the real coverage in the MaSurCa genome assembly?.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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