If I assemble the RNA-Seq data de novo,how can I estimate the transcription level?
My first thought is to calculate the reads frequences of 3' ends,although it seems arbitrary.I use Velvet,generated .afg file may contain useful infomation,but how to analyze this file?Is there any good suggestions?
My first thought is to calculate the reads frequences of 3' ends,although it seems arbitrary.I use Velvet,generated .afg file may contain useful infomation,but how to analyze this file?Is there any good suggestions?
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