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  • Primer3 : oligonucleotide determining

    Hello ! I would like to determine oligonucleotides of housekeeping (1) and target (2) genes with Primer3web.
    I have these mRNA sequences :
    (1) CTCCCGATCAGCAGCGTCTTATCTTCGCTGGCAAGCAACTTGAGGATGGTCGCACTCTCTCAGACTACAA
    CATCCAGAAGGAATCCACTCTCCATCTCGTTCTCCGACTTCGTGGTGGTATGCAGAGTGTATTAAGATAT
    AGATGGACAGTCCAATATTATTCTATATTTCTCATAAATGACAAGCAAGTCTATTATATTCTAAACAAAT
    TAATGTGGCTTTATCTTATCAAGAGAAGAAGTGCACCCATCAATACAAGAAGAATCGTATCAAGATTGCA
    GAG
    (2) AACCTGAGCACCACCCCGTTTGGCCGTGCGCTGTATACCCTGAGCAGCGATACCCAGTGGATTGATCAGC
    GTCTGGTGCATACCTTTAGCATGCTGCTGAACACCAGCGTGGCGGTGATTGCGAGCTTTATTGTGAACGC
    GATTGTGAACGTGTATTTTATTATTTTTCTGATTCCGATTGGCTTTGTGTTTTTTGGCATTCTGGTGTAT
    TTTGTGGCGACCAGCCGTATGCTGCAGCGTATTGAAAGCGTGACCCGTAGCCCGGTGTTTGCGCATTTTA
    GCGAAACCCTGGGCGGCCTGCCGATTATTCGTGCGTATCGTGATGAAAAACGTTTTTTTAAAAACATTCT
    GGCGAAAATTGATAAAAACAACACCGCGCTGGTGTATCTGTATACCGCGAACCGTTGGCTGGCGATTCGT
    CTGGATTATCTGGGCGCGGTGATTGTGCTGGTGGCGAGCCTGAGCGCGCTGCTGGGCGCGTTTTATCTGG
    GCGTGGATGCGAGCCTGGTGGGCCTGGCGATTACCTATAGCCTGGAAATTAGCATTTATATGAACATGGT
    GGTGCGTGGCTGCGCGGATCTGGAACTGCAGATGAACGCGATGGAACGTATTCGTTATTATACCGAAGTG
    CCGAACGAAGAATATGAAGGCCTGGAACCGCCGCCGGAATGGCCGCATAAAGGCGAAATTATTCTGGATA
    ACATTAGCGTGCGTTATGCGGCGGAACTGGATCCGGTGCTGCCGCTGAGCGGCTATTTTAGCCCGGGCGG
    CTTTGGCTGGTATAGCGTGCTGCATACCGGCAGCGGCAAAAGCAGCTTTACCCTGGCGCTGTATCAT
    Can you help me to choose parameter values (see attach file) please ?
    Thank you !
    Attached Files

  • #2
    Please don't post the same question in multiple forums. I have deleted the duplicates.

    Comment


    • #3
      Sorry for the duplicates. This is the first time I use this forum and I did not known which place between "RNA Sequencing", "General" and "Bioinformatics" was the best.
      Thank you for you comprehension.

      Comment

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