I have used Primer3 tool within Geneious to design a large number of primers. There is one parameter that I would like some help in interpreting. The primer dimer score, which ranges from 0 to a large number of some kind, is somewhat confusing. How is this calculated? What is considered a “good” number for primer dimers not to form? Obviously zero is ideal, but when I look at my dataset, It ranges up to 30 or so, with everything in between. The online help information gives examples of 6 or 7. I would appreciate any laboratory experience advice on what would be a good threshold to avoid. I am developing primers for Fluidigm/Illumina. Thanks!
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...-
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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