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Old 05-01-2017, 11:53 AM   #1
Joseph White
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Location: USA

Join Date: Jun 2016
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Default Baits in WGS???

Hi,

Capture baits are used for exome sequencing in WES. Are baits used for whole genome sequencing. Or, is sequencing randomly primed for WGS?

Thanks for any info.
Cheers,
jwhite
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Old 05-01-2017, 04:26 PM   #2
nucacidhunter
Jafar Jabbari
 
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Common method for WGS library prep is ligating adapters to randomly sheared DNA. There are also less common methods such as tagmentation that involves less randomly cutting DNA and inserting a sequence overhang for subsequent PCR amplification. Although technically incorrect, you can think of these methods as randomly primed sequencing.
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