Hi,
I'm wondering whether anyone here has ever succeeded using Bioruptor Pico sonicator for transcription factor ChIP-qPCR.
Before, we had an old standard Bioruptor which worked okay with both ChIP-qPCR and ChIP-seq. Recently, we've switched to the new product from Diagenode, i.e. Bioruptor Pico. When we look at the input sample on agarose gel, it suggests that the new sonicator is very efficient for chromatin shearing (if using their special tubes from Diagenode).
However, I am currently unable to perform a successful ChIP-qPCR (no enrichment, Ct of ChIP samples is very very high).
I'm sure both the protocol and antibodies are fine, as we have been doing ChIP-qPCR and ChIP-seq for years without any problem using the same procedure, except, of course, the sonicator.
I ran my input sample and IPs on western blot, the strange thing is that I cannot even detect my TF in the input sample ......
I am wondering whether anybody here has the same problem, or anybody is using this sonicator without any problems.
Thank you.
I'm wondering whether anyone here has ever succeeded using Bioruptor Pico sonicator for transcription factor ChIP-qPCR.
Before, we had an old standard Bioruptor which worked okay with both ChIP-qPCR and ChIP-seq. Recently, we've switched to the new product from Diagenode, i.e. Bioruptor Pico. When we look at the input sample on agarose gel, it suggests that the new sonicator is very efficient for chromatin shearing (if using their special tubes from Diagenode).
However, I am currently unable to perform a successful ChIP-qPCR (no enrichment, Ct of ChIP samples is very very high).
I'm sure both the protocol and antibodies are fine, as we have been doing ChIP-qPCR and ChIP-seq for years without any problem using the same procedure, except, of course, the sonicator.
I ran my input sample and IPs on western blot, the strange thing is that I cannot even detect my TF in the input sample ......
I am wondering whether anybody here has the same problem, or anybody is using this sonicator without any problems.
Thank you.
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