We are just preparing our lab for the installation of a HiSeq. We are in an older building so have some concern about the +/- 2C temperature requirements during the length of the run. I am wondering if anyone has any comments on how important this temperature control is.
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We were told by the Illumina engineers that there wasn't a temperature requirement for the HiSeq since the flowcell plate is actively temperature regulated (as opposed to our GA2, which has a peltier unit that comes on and off).
Disclaimer: This is what our lab manager told me.Last edited by Lee Sam; 08-16-2010, 11:17 AM.
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From what our FAS has told us, 68F (20 c) is the sweet spot for the HiSeq's; this isn't as important with respect to the flow cell as it is with respect to the lasers/other components. I personally start getting nervous when I start seeing the ambient temp edging into 69, and if it were to ever hit 71 I'd call facilities.
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