Go Back   SEQanswers > Applications Forums > RNA Sequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
Multiplexing RNA-Seq and ChIP-Seq in same lane menzies Illumina/Solexa 3 07-27-2012 08:00 AM
Sequencing mouse and homan mixed sample in one lane (or one barcode) by RNA-Seq? chipgal Illumina/Solexa 1 06-27-2012 07:20 AM
Best HiSeq lane ever? pmiguel Illumina/Solexa 2 04-30-2012 06:20 AM
small rna seq with total lane read count different between conditions anle Bioinformatics 0 01-25-2012 01:25 PM
When can I get away without a control lane? lparsons Illumina/Solexa 4 11-02-2009 12:37 AM

Thread Tools
Old 01-10-2013, 06:28 PM   #1
Pengfei Liu
Location: Beijing

Join Date: Nov 2012
Posts: 16
Unhappy one lane or two lane in RNA-seq

hi all,
I have RNA-seq project with 3 treatment and 4 replicates per treat, my supervisor want me to use MiSeq. But i find Miseq only get 7.5-8.0G data per lane, so I have to use run on two lanes for my samples. If I use HiSeq 2000, one lane could be enough.

I don't know if this will affect the results and reliability of the data if run on two lanes or two run?

Pengfei Liu is offline   Reply With Quote
Old 01-11-2013, 04:41 AM   #2
Senior Member
Location: Boston,MA

Join Date: Nov 2008
Posts: 122

One or two lanes will not affect your data as it is all sequence-not intensity based.

You can continue to run as many lanes as you want and continue to add data.

I will tell you that in my experience, that unless you are working with a very small organism, you will need ffar more data than even one lane of HiSeq will provide. For human/mouse, we generally run only 4 samples per HiSeq lane.
kwaraska is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 03:45 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO