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Old 01-13-2013, 06:20 PM   #1
Pengfei Liu
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Location: Beijing

Join Date: Nov 2012
Posts: 16
Question RIN of bacteria and archaea

Hi,
I am going to run a RNA-seq of prokaryotes (a bacteria strain, an archaea strain and the combination of the two). Now I am confused about the RIN number of the prokaryotes. Form reading paper and the knowledge in this forum, RIN is used to indicated the intergrity of eukaryotes' RNA. So, the RIN large than 7.5 or 8 were suggestes for RNA-seq, but the locoal sequencing center told me for bacteria, 6.0 or larger is OK for prokaryotes.

And for the determine of prokaryotes RIN, is there anything I should notice which is different from the calculation of eukaryotes?

who had experience on this issue, please give me some advises, thank you very much.
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