>ILLUMINA-053F9F_0003:8:1:1051:4272#0/1 GTTTTAATCGATAACGGGAGATGTGGTGTTCGGGAGGACCCGCTTCACACTAATAAAGCGTAACCCTACAATCCAAACAATCTCG 2:0:0 silkworm2.fa/scaffold1100:8972F37TT6TCTCG1CGCG1C1AC1CCTGTCCA1G1T1GTCTTGAACG1,silkworm2.fa/scaffold1143:2756F37TT6TCTCG1CGCG1C1AC1CCTGTCCA1G1T1GTCTTGAACG1
I want to know that
1) what does the last string after '2756F', that is '7TT6TCTCG1CGCG1C1AC1CCTGTCCA1G1T1GTCTTGAACG1', mean?
2) Actually only the first 37bp of this read (85bp) can align the reference by my blast verification, so why eland report it as mapped with less than 2 errors?
Query: 1 gttttaatcgataacgggagatgtggtgttcgggagg 37
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Sbjct: 2756 gttttaatcgataacgggagatgtggtgttcgggagg 2792
I want to know that
1) what does the last string after '2756F', that is '7TT6TCTCG1CGCG1C1AC1CCTGTCCA1G1T1GTCTTGAACG1', mean?
2) Actually only the first 37bp of this read (85bp) can align the reference by my blast verification, so why eland report it as mapped with less than 2 errors?
Query: 1 gttttaatcgataacgggagatgtggtgttcgggagg 37
|||||||||||||||||||||||||||||||||||||
Sbjct: 2756 gttttaatcgataacgggagatgtggtgttcgggagg 2792
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