Hi,
Could anyone help me learn how to remove an unknown primer sequence from the ends of reads. I have these primers attached to the DNA prior to making the Illumina libraries as i have used them for other reactions. This means that when the data is compiled i have a lot of data that is unmappable due to these primers.
Any ideas?
I have to admit...i'm no bioinformatician so could you keep your replies easy for me to understand please.
Thanks in advance
Could anyone help me learn how to remove an unknown primer sequence from the ends of reads. I have these primers attached to the DNA prior to making the Illumina libraries as i have used them for other reactions. This means that when the data is compiled i have a lot of data that is unmappable due to these primers.
Any ideas?
I have to admit...i'm no bioinformatician so could you keep your replies easy for me to understand please.
Thanks in advance
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