Hi All,
I'm new to RNA-Seq, so I'm not that familier with RNA quality checks for RNA seq. I have 6 total bacterial RNA samples, that I want to carry for rRNA depletion using the RiboZero kit. Before doing that I ran the samples on agarose gel and got a strange result (please see attached). It seems that all the samples have a huge band around 100bp.. what this means? is that degraded RNA? or maybe DNaseI degraded DNA leftovers?
Any help interpreting this gel will be highly appreciated!
Thanks and best,
Lior
I'm new to RNA-Seq, so I'm not that familier with RNA quality checks for RNA seq. I have 6 total bacterial RNA samples, that I want to carry for rRNA depletion using the RiboZero kit. Before doing that I ran the samples on agarose gel and got a strange result (please see attached). It seems that all the samples have a huge band around 100bp.. what this means? is that degraded RNA? or maybe DNaseI degraded DNA leftovers?
Any help interpreting this gel will be highly appreciated!
Thanks and best,
Lior
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