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Nat Methods. 2010 Feb;7(2):119-22. Epub 2010 Jan 17.
Parallel, tag-directed assembly of locally derived short sequence reads.
Hiatt JB, Patwardhan RP, Turner EH, Lee C, Shendure J.
[1] Department of Genome Sciences, University of Washington, Seattle, Washington, USA. [2] These authors contributed equally to this work.
We demonstrate subassembly, an in vitro library construction method that extends the utility of short-read sequencing platforms to applications requiring long, accurate reads. A long DNA fragment library is converted to a population of nested sublibraries, and a tag sequence directs grouping of short reads derived from the same long fragment, enabling localized assembly of long fragment sequences. Subassembly may facilitate accurate de novo genome assembly and metagenome sequencing.
PMID: 20081835
Parallel, tag-directed assembly of locally derived short sequence reads.
Hiatt JB, Patwardhan RP, Turner EH, Lee C, Shendure J.
[1] Department of Genome Sciences, University of Washington, Seattle, Washington, USA. [2] These authors contributed equally to this work.
We demonstrate subassembly, an in vitro library construction method that extends the utility of short-read sequencing platforms to applications requiring long, accurate reads. A long DNA fragment library is converted to a population of nested sublibraries, and a tag sequence directs grouping of short reads derived from the same long fragment, enabling localized assembly of long fragment sequences. Subassembly may facilitate accurate de novo genome assembly and metagenome sequencing.
PMID: 20081835