Hi,
I am interested in sequencing 40 strains of yeast ( ~12Mb)
genome size. Illumina provides 10 validated barcodes but I need
30 more. I would be grateful if people can share their custom
(and validated) barcode sequence or provide any advice
on designing our own barcodes that will not interfere with illumina
sequencing reaction. Reading through some previous posts on
multiplexing, one advice I gathered was that the first four
bases of the bar code is used by the illumina software for cluster
identification--but that leaves only 16 unique barcodes which
is insufficient.
thanks in advance
Christoph
I am interested in sequencing 40 strains of yeast ( ~12Mb)
genome size. Illumina provides 10 validated barcodes but I need
30 more. I would be grateful if people can share their custom
(and validated) barcode sequence or provide any advice
on designing our own barcodes that will not interfere with illumina
sequencing reaction. Reading through some previous posts on
multiplexing, one advice I gathered was that the first four
bases of the bar code is used by the illumina software for cluster
identification--but that leaves only 16 unique barcodes which
is insufficient.
thanks in advance
Christoph
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