Hi all,
I would like to target a ~10 kb region for NGS. I was looking at a paper by Myllykangas et al. (http://www.biomedcentral.com/1472-6750/11/122/) which circularizes a target region and prepares it for Illumina sequencing, using very short (~40 bp) fragments.
I have other reasons for wanting to circularize the sequence and keep it intact prior to sequencing, and was wondering if this is possible for such a large fragment. Any suggestions/references for protocols to target and circularize large fragments would be greatly appreciated.
I would like to target a ~10 kb region for NGS. I was looking at a paper by Myllykangas et al. (http://www.biomedcentral.com/1472-6750/11/122/) which circularizes a target region and prepares it for Illumina sequencing, using very short (~40 bp) fragments.
I have other reasons for wanting to circularize the sequence and keep it intact prior to sequencing, and was wondering if this is possible for such a large fragment. Any suggestions/references for protocols to target and circularize large fragments would be greatly appreciated.
Comment