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Old 04-06-2012, 10:25 AM   #1
bernieball
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Location: North Carolina

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Default Bioruptor shearing of PCR product

We are pooling multiple PCR products that range in size from 700 to 2000 bp and would like to fragment them to approx. 450-500 bp before tagging them with barcodes.

Has anyone worked with PCR products vs. genomic DNA in a similar manner?

We will be using a Bioruptor UCD-200.

Thanks for your help!
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Old 11-25-2013, 06:37 AM   #2
jchung
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Bernie,

I'm curious to know how your shearing worked for those mixed amplicon sizes. We are trying to develop a shearing protocol as well for amplicon pools of 300bp - 2kb.

Thanks!
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