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Old 06-22-2012, 04:13 AM   #1
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Location: Cardiff

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Default bwa index from reference with unusual chromosomes

A similar question to

I'm about to create my reference index for aligning some exome data. I wonder should I includes these chromosomes in the reference index. At first I thought "what could be the harm" but I will be using GATK downstream, and GATK doesn't work if the chromosomes are out of order. How then should I handle these non-canonical chromosomes when there is no implicit order.

Thanks for any advice
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Old 06-22-2012, 03:04 PM   #2
Nils Homer
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Use the 1000 genomes reference, and you should have no problems..
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ambiguous chromosomes, bwa index, reference genome

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