I am using the Illumina TruSeq ChIP Library prep kit to generate a library following chromatin immunoprecipitation. I will then sequence 100 bp, paired-end reads on the Illumina HiSeq platform. Before adapter ligation, most of my DNA fragments had a length of 200-250 bp.
What size should I cut out and recover following the adaptor ligation and PCR amplification? Thank you.
What size should I cut out and recover following the adaptor ligation and PCR amplification? Thank you.
Comment