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Old 11-04-2014, 08:08 PM   #1
aliceli
Junior Member
 
Location: Taipei

Join Date: Jun 2012
Posts: 2
Default ligation problem for pcr product

We have 10k pcr product to construct the pacbio library. The original and concentrate DNA look good on bioanalyzer, and the band is pure 10kb band. However, the library is smear from 3kb to 10kb. please refer to the attach file. Why the DNA degrade? We don't even use vortex to treat DNA. Does anyone have the experience like that?
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