Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
ATAC-seq (Read1/Read2/Index Sequencing Primers?) emacalate Epigenetics 1 08-30-2016 11:11 AM
ATAC sequencing sample dataset balaszone Bioinformatics 1 06-14-2016 12:34 AM
merging sequencing data from different sequencing runs csoong Bioinformatics 15 05-15-2013 11:02 AM
How to merge multiple sequencing runs vinay052003 Bioinformatics 4 01-31-2012 03:34 AM

Thread Tools
Old 04-23-2018, 05:51 AM   #1
Libby Evans
Junior Member
Location: Oxford

Join Date: Apr 2018
Posts: 1
Default Balance sequencing runs ATAC

I am having trouble balancing my sequencing runs for ATAC-seq. I am doing extensive kapa on the samples but often finding one out of a pool of 6 will be far too high (on one occasion taking 40% of the total reads). These samples often look quite low by kapa. Has anyone experienced this?

If you do size correction how do you do this? (On the tapestation or bioanalyser?) Also do you typically do a size selection after the indexing clean up? I haven't included that yet but could. Any advice very welcome! Thanks.
Libby Evans is offline   Reply With Quote

atac, atac-seq, library, quantification

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 01:43 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO