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Old 01-09-2018, 03:03 PM   #1
hoytpr
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Default If you use IEM, watch out for A027

We just completed a sequencing run that used the Illumina A027 (or the AR027) index on one of the samples. Setting it up on the NEWER version of IEM incorrectly assigns it the sequence of ATTCTT, but the correct sequence, (and the one assigned by the older IEM software) is ATTCCT.

Luckily the Bcl2Fastq detected the 99+% "one basepair mismatch barcode" and all the samples sorted properly, but y'know, a bug's a bug.

Last edited by hoytpr; 01-09-2018 at 03:20 PM. Reason: added index number
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Old 01-10-2018, 04:03 AM   #2
GenoMax
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Hopefully you have reported this back to Illumina so IEM can be fixed.
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Old 01-10-2018, 06:10 AM   #3
hoytpr
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Yes, and it was confirmed yesterday to be a bug in IEM 1.14 and 1.15 (just released), but not in 1.11. I have not tested 1.12, or 1.13.
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