Hi everyone.
I just trying to deal with the work started for one of the master students in my new lab. I'm now dealing with a set of 64 bacterial draft genomes, and the first thing that I need to understand is if the quality of the assembly is good or not.
I have a huge list of different statistics, that I supposed were obtained by using Velvet for genome assembly. These statistics include the following:
- Read length
- Total read length (bp)
- Total read length (Mb)
- Estimated coverage
- Velvet hash value
- Total number of sequences
- Total number of contings
- N50
- Length lof longest contig
- Total bases in contigs
- Number of consigns > 1K
- Total bases in contigs > 1K
Can any one explain me what's the meaning of each statistic and where I should relay most to evaluate the quality of the obtained draft genome??
I'm completely lost!
Thank you very much in advance
I just trying to deal with the work started for one of the master students in my new lab. I'm now dealing with a set of 64 bacterial draft genomes, and the first thing that I need to understand is if the quality of the assembly is good or not.
I have a huge list of different statistics, that I supposed were obtained by using Velvet for genome assembly. These statistics include the following:
- Read length
- Total read length (bp)
- Total read length (Mb)
- Estimated coverage
- Velvet hash value
- Total number of sequences
- Total number of contings
- N50
- Length lof longest contig
- Total bases in contigs
- Number of consigns > 1K
- Total bases in contigs > 1K
Can any one explain me what's the meaning of each statistic and where I should relay most to evaluate the quality of the obtained draft genome??
I'm completely lost!
Thank you very much in advance
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