Hi all,
We are looking to sequence a 100 kb region of the human genome for 400 samples. We are not sure what is the current recommended method for amplifying a region of this size for illumina sequencing.
We are looking into Illumina Tru seq custom amplicon kit, but would this be possible to do with a more conventional long range pcr by designing primer for large regions (such as 20KB each), preping that DNA and then sending it off?
Any input would be of help.
Thanks
We are looking to sequence a 100 kb region of the human genome for 400 samples. We are not sure what is the current recommended method for amplifying a region of this size for illumina sequencing.
We are looking into Illumina Tru seq custom amplicon kit, but would this be possible to do with a more conventional long range pcr by designing primer for large regions (such as 20KB each), preping that DNA and then sending it off?
Any input would be of help.
Thanks
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