Hi all,
I want to use 300 cycle reagent kit from illumina with MiSeq
So, My questions are:
- Can I choose single read from Illumina Experiment Manager and read 300 bases from one direction instead of 150 from each side in Paired end read situation?
-Is there any negative effect on reads if I did that? like the quality will be low at the last 50 or 100 bases of the read for example.
-Doing that doesn't have any relation with using dual-indexing, am I right?
I hope my questions are clear and I get a reply from anyone of you.
Many Thanks
Suha
I want to use 300 cycle reagent kit from illumina with MiSeq
So, My questions are:
- Can I choose single read from Illumina Experiment Manager and read 300 bases from one direction instead of 150 from each side in Paired end read situation?
-Is there any negative effect on reads if I did that? like the quality will be low at the last 50 or 100 bases of the read for example.
-Doing that doesn't have any relation with using dual-indexing, am I right?
I hope my questions are clear and I get a reply from anyone of you.
Many Thanks
Suha
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