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  • MapSplice error

    hello,
    I am running MapSplice and I am able to get the output files, but there is empty in my best_junction.bed file.

    Furthermore, I got some error messenger form MapSplice:

    [Thu Jun 28 04:07:56 2012] Beginning Mapsplice run (v1.15.2)
    -----------------------------------------------
    bin directory: [/home/biongs158/Mapsplice_1.15.2/bin/]
    [Thu Jun 28 04:07:56 2012] Checking for chromosomes files or directory
    [Thu Jun 28 04:07:56 2012] Checking for chromosomes files or directory passed
    [Thu Jun 28 04:07:56 2012] Checking for Bowtie index files
    [Thu Jun 28 04:07:56 2012] check reads format
    [Thu Jun 28 04:08:43 2012] merge paired end reads remove short
    [Thu Jun 28 04:15:14 2012] Mapping reads against SRS000047_chr11_no_n with Bowtie
    [Thu Jun 28 04:20:06 2012] Converting bowtie mapped to SAM format
    [Thu Jun 28 04:20:52 2012] divide reads
    [Thu Jun 28 04:36:48 2012] Mapping reads against SRS000047_chr11_no_n with Bowtie
    [Thu Jun 28 04:50:55 2012] sort segmentbwt
    [Thu Jun 28 05:00:54 2012] reads all chromo sizes
    [Thu Jun 28 05:00:56 2012] mapsplice_search
    [Thu Jun 28 05:14:51 2012] Aligning spliced reads
    [Thu Jun 28 05:28:32 2012] mapsplice_report
    [Thu Jun 28 05:35:56 2012] remove index of sam
    [Thu Jun 28 05:35:59 2012] sort segmentbwt
    [Thu Jun 28 05:36:01 2012] convert sam files to junctions
    terminate called after throwing an instance of 'std::length_error'
    what(): basic_string::_S_create

    [Thu Jun 28 05:36:03 2012] sort by name
    [Thu Jun 28 05:36:33 2012] Remove duplication of sam
    [Thu Jun 28 05:37:09 2012] Filtering junctions
    [Thu Jun 28 05:37:09 2012] Remove duplication of sam
    [Thu Jun 28 05:37:12 2012] Filter Reads By Canon Noncanon
    [Thu Jun 28 05:37:12 2012] convert sam files to junctions
    terminate called after throwing an instance of 'std::length_error'
    what(): basic_string::_S_create

    [Thu Jun 28 05:37:13 2012] convert sam files to junctions
    terminate called after throwing an instance of 'std::length_error'
    what(): basic_string::_S_create

    [Thu Jun 28 05:37:14 2012] filter junction by min mis and min lpq
    [Thu Jun 28 05:37:14 2012] filter junc by ROC argu noncanonical
    [Thu Jun 28 05:37:14 2012] filter junction by min mis and min lpq
    [Thu Jun 28 05:37:14 2012] convert junction file to bed format
    [Thu Jun 28 05:37:15 2012] Filter Sam By junction
    [Thu Jun 28 05:37:16 2012] Merging all sam files
    [Thu Jun 28 05:37:20 2012] convert sam files to junctions
    terminate called after throwing an instance of 'std::length_error'
    what(): basic_string::_S_create

    [Thu Jun 28 05:37:26 2012] convert junction file to bed format
    [Thu Jun 28 05:37:26 2012] sort segmentbwt
    [Thu Jun 28 05:37:34 2012] Add tags to sam file
    [Thu Jun 28 05:47:31 2012] combine to final sam

    [Thu Jun 28 05:49:16 2012] Finishing Mapsplice run (time used: 1:41:20.265268)

    Does anyone have these error?
    Hope you can help me,thanks a lot!
    Last edited by sean760305; 06-28-2012, 01:31 AM.

  • #2
    Please email the author, I think he will be glad to help you.

    Comment

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