Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • velvet denovo

    Hi ..I was running velvetg with various parameters but for different coverege cutoff iam getting the different results...how to optimize the coverege cutoff ..


    thanks

  • #2
    In the manual there is a description of how generate an histogram that can help you in decide several parameters.
    In general the best way to proceed is make some test on a little data set. You keep the parameters that give the best result on the small data set and you use them on the whole data set

    Francesco

    Comment


    • #3
      you should also look at N50, read usage, and the count of 1k contigs

      the script in my sig might help you
      --
      Jeremy Leipzig
      Bioinformatics Programmer
      --
      My blog
      Twitter

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin




        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
        04-22-2024, 07:01 AM
      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Yesterday, 11:49 AM
      0 responses
      15 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-24-2024, 08:47 AM
      0 responses
      16 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      61 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      60 views
      0 likes
      Last Post seqadmin  
      Working...
      X