Hi, there!
I have used pacbio SMRT Iso-seq module to analyze my cDNA sequencing data, including Getting full length reads, Isoform level clustering (ICE and Quiver), Removing redundant transcripts. And I have got the isoform cluster result. Then I want to do the SNP , gene family or fusion genes analysis, but most of the publications show that this must be accompanied with the short-read sequencing data(eg. HiSeq data). So is there any analysis method can do with only pacbio Iso-seq data?
Thanks a lot for any apply
I have used pacbio SMRT Iso-seq module to analyze my cDNA sequencing data, including Getting full length reads, Isoform level clustering (ICE and Quiver), Removing redundant transcripts. And I have got the isoform cluster result. Then I want to do the SNP , gene family or fusion genes analysis, but most of the publications show that this must be accompanied with the short-read sequencing data(eg. HiSeq data). So is there any analysis method can do with only pacbio Iso-seq data?
Thanks a lot for any apply
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