Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • PE Sequencing or wait 1 year for costs do go down?

    Hi,

    we are planning our new sequencing experiment and are arguing about single end reads against paired end reads.

    If we sequence 100bp PE reads instead of 100bp SE reads it would cost ~70% more. So our/my main question is:

    Do you thing it's worth it to sequence PE reads, ie. is the gain in information worth the extra money?

    As we have limited human resources, I think analyzing the data will last at least a year. Do you think by then it is cheaper to sequence it with PE reads again as the cost are going down, ie. could we get it done for the same prize = the 70% we are would now pay extra?


    Any suggestions would be highly appreciated!!

    All the best!

  • #2
    It would help to know what your experiment/application was in order to ascertain whether you would gain information with a PE approach..

    Comment


    • #3
      If you really plan to wait a year you're almost guaranteed the price will drop at least close to 70% and possibly more. What type of project is this?

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM
      • seqadmin
        Techniques and Challenges in Conservation Genomics
        by seqadmin



        The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

        Avian Conservation
        Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
        03-08-2024, 10:41 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Yesterday, 06:37 PM
      0 responses
      11 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, Yesterday, 06:07 PM
      0 responses
      10 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-22-2024, 10:03 AM
      0 responses
      51 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-21-2024, 07:32 AM
      0 responses
      68 views
      0 likes
      Last Post seqadmin  
      Working...
      X