Hello experts,
I have spent whole day trying to upload my Illumina paired-end reads to the NCBI SRA. I have read through the manual over and over but it is quite confusing. Could anyone help me figure out how to solve my problems? Any advice would be greatly appreciated.
I have created one BioProject and one BioSample. The BioSample contains 8 samples.
1. When I create a new submission in the SRA, should I create an experiment and a run for each sample? Or should I create only one experiment and create 8 different runs under one experiment?
2. For Illumina paired-end reads, can I upload an interleaved file for each sample or should it be two separate files (R1 and R2)?
3. I tried to upload two separate files (R1 and R2) which were quality trimmed for one sample. The upload failed. Should I upload only raw sequences or assembled contigs?
I have spent whole day trying to upload my Illumina paired-end reads to the NCBI SRA. I have read through the manual over and over but it is quite confusing. Could anyone help me figure out how to solve my problems? Any advice would be greatly appreciated.
I have created one BioProject and one BioSample. The BioSample contains 8 samples.
1. When I create a new submission in the SRA, should I create an experiment and a run for each sample? Or should I create only one experiment and create 8 different runs under one experiment?
2. For Illumina paired-end reads, can I upload an interleaved file for each sample or should it be two separate files (R1 and R2)?
3. I tried to upload two separate files (R1 and R2) which were quality trimmed for one sample. The upload failed. Should I upload only raw sequences or assembled contigs?
Comment