Hello,
I am working on 24 Human gut microbiome samples generated via illiumina hiseq, almost 800 gb data.
I have optimized and assembled the data using velvet but on different K mer, having assembly size; varying from 35 to 115mb. My query is there any need of justification to assemble all data on different k-mers. Is it ok or i should go for only one K-mer based assembly, also how do I proceed to assembly normalization.
I am working on 24 Human gut microbiome samples generated via illiumina hiseq, almost 800 gb data.
I have optimized and assembled the data using velvet but on different K mer, having assembly size; varying from 35 to 115mb. My query is there any need of justification to assemble all data on different k-mers. Is it ok or i should go for only one K-mer based assembly, also how do I proceed to assembly normalization.
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