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Old 01-17-2013, 11:14 AM   #1
mm.perrineau
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Location: New Jersey

Join Date: Sep 2012
Posts: 12
Default I will be crazy: Help about SNPs and tree!!!!

Hi,

Somebody can help me????
I will be crazy: I am not bioinformaticien and i have to make a phylogenetic tree reflecting the genetic distance between 4 strains...
I have one strain well sequenced and his genome is annotated...
I have all reads mapped on this genome for the 3 others strains...

I used CLC for the SNPs calling and now i am stopped since few weeks and i become crazy!!!!!!!!
I need to build a simple tree reflecting the genetic distance between those 4 strains...
Can you help me...

And i need too some software to make some statistical tests on the SNPs... Do i have some genomic regions ? some genes under selection pressure ?

Thank you very much !!!!!

And please help me i am totally desperate !!!!
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Old 06-14-2013, 08:39 PM   #2
khoa128
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Location: hanoi

Join Date: Sep 2012
Posts: 2
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Hi
you to be used software of this: NextGENe® software for Next Generation (NGS) Sequence Analysis. You may download a 30 day trial from our ftp site:

ftp://ngval:YP5NM7SZ@ftp.softgenetic...ion_35Days.EXE
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Old 08-07-2013, 09:42 AM   #3
AdrianP
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Location: Ottawa

Join Date: Apr 2011
Posts: 130
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Hello,

I do not mean to advertise my own thread, but it seems like we are doing something similar. Check out what I have done so far and my obstacles:

http://seqanswers.com/forums/showthread.php?p=112840
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